macro - What are the best practices for DOF stacking?

What are the best practices for DOF stacking? How do you get the frames with focus just right amount apart? How do you stack them in post processing?

Answer

If you are taking photos of something stationary, then a focusing rail will allow you to do them perfectly. If you are talking about moving things, like insects, then all you can do is take a lot of photos. If you aren't able to focus reliably without a tripod and rail, then you can use burst mode. But, with some practice, you can get to the point where you can reliably click the shutter when specific portions of the shot are in focus and capture a series that way instead.